复方健胰颗粒对代谢综合征大鼠超敏C反应蛋白和主动脉组织形态学的影响

Effects of Fufangjianyi Granule on High-sensitivity C-reactive Protein and Aortic Morphology in Metabolic Syndrome Rats

目的观察复方健胰颗粒对代谢综合征大鼠超敏C反应蛋白和主动脉组织形态学的影响。方法将SD大鼠随机分为正常对照组、代谢综合征组、罗格列酮治疗组、复方健胰颗粒治疗组、复方健胰颗粒预防组。采用高脂饲料+腹腔注射维生素D3诱导代谢综合征模型,尾部加压法测量血压,生化法检测血脂、血糖、超敏C反应蛋白,放免法测空腹胰岛素,HE染色观察主动脉组织形态学的变化。结果复方健胰颗粒治疗组、复方健胰颗粒预防组较代谢综合征组体重、血压、血糖、血清胰岛素、超敏C反应蛋白水平降低,同时改善了大鼠脂质代谢。复方健胰颗粒治疗组较代谢综合征组主动脉内膜损伤较轻,斑块较小、较薄,中膜层弹力纤维稍增厚、排列有序,平滑肌细胞增生不明显,泡沫细胞及脂质沉积明显减少;复方健胰颗粒预防组主动脉内、中膜未见明显异常。结论复方健胰颗粒可降低超敏C反应蛋白水平,可预防和治疗由高脂饲料+腹腔注射维生素D3诱导的大鼠动脉粥样硬化。

Objective To study the effects of Fufangjianyi granule on hs-CRP and aortic morphology in the rats model of metabolic syndrome. Methods SD rats were randomly divided into normal control group, metabolic syndrome group, rosiglitazone treatment group, Fufan^ianyi granules treatment group, Fufangjianyi granules prevention group. High fat diet and intraperitoneal injection of vitamin D3 was used to induce metabolic syndrome model. Blood pressure was measured by taft-pressure method. Blood lipids, blood sugar, hs-CRP were detected by biochemical method. Fasting plasma insulin was measured by radioimmunoassay, and histological changes of aorta was observed by HE staining. Results Compared with metabolic syndrome group, body weight, blood pressure, blood glucose, serum insulin, hs-CRP levels in Fufangjianyi granules treatment group and prevention group decreased, at the same time lipid metabolism was improved. Compared with model group, aorta injury of Fufan^ianyi granules treatment group was lighter, patches were thinner and smaller, the film was slightly thickened elastic fibers arranged orderly, smooth muscle cell proliferation was not obvious, foam cells and lipid mass deposition significantly reduced. There were no obvious abnormalities in the aortic membrane of Fufan^ianyi granules prevention group. Conclusion Fufangjianyi granules can reduce hs-CRP levels, prevent and treat high fat diet and intraperitoneal injection of vitamin D3-induced atherosclerosis in rats.