耐高温、高浓度酒精酵母高密度培养条件优化

Optimization of high cell density cultivation conditions of thermotolerant and ethanol tolerant yeast

S132是一株优良的耐性酒精酵母菌株,试验以YPD培养基为基础,通过单因子、中心组合设计及正交试验设计优化培养基组成及培养工艺,确定了影响S132生长的最重要因子为葡萄糖,最适培养基条件为:葡萄糖90g/L,酵母粉13.1g/L,蛋白胨10.6g/L,培养温度30℃,接种量2×106cfu/mL,转速175r/min,pH值为4.8,此条件下菌落数可达4.0×108cfu/mL,比优化前提高140%。50L发酵罐小试试验显示,S132罐培养12h即进入稳定期,菌落数可达4.1×108cfu/mL。

S132 is good alcohol-tolerant yeast. Based on the YPD medium, the fermentation conditions were optimized by single factor analysis, central composite design and orthogonal design method. The results showed that the important component influencing the cell density was glucose. The optimal fermentation conditions were as followed： glucose 90g/L, yeast extract 13.1g/L, peptone 10.6g/L, fermentation temperature 30℃, agitation speed 175r/min, pH value 4.8 and the inoculum 2×10 6cfu/ml. Under these conditions, the cell density could reach 4.0×10 8cfu/ml, which was increased by 140% compared with that of the control. The results of 50L pilot test indicate that the S132 enter stationary phase after 12h fermentation and the cell density reached 4.1 × 10 8cfu/ml.