明胶载体固定化乙醇脱氢酶技术研究

Immobilization of alcohol dehydrogenase in gelatin

以明胶为载体,戊二醛为交联剂,采用包埋-交联联用法制备了明胶固定化乙醇脱氢酶。结果表明:以15%明胶为载体、4%戊二醛为交联剂、1g明胶固定1mL乙醇脱氢酶制备的固定化酶,其酶活力回收率达52.47%,所得固定化乙醇脱氢酶连续使用10次后相对活力为52.89%。

Alcohol dehydrogenase was immobilized with gelatin and cross linked with glutaraldehyde. The results showed that alcohol dehydrogenase activity was 52.47% when lml alcohol dehydrogenase was immobilized with 15% gelatin, 4% glutaraldehyded and lg gelatin. The recovery activity of immobilized pectinase was 52.89% after repeated for 10 times.