番茄HsfA3基因功能验证中相关植物表达载体的构建

Construction of plant expression vectors of tomato HsfA3 gene for functional analysis

以pBI121质粒为基本载体,分别构建了由35S启动子驱动的用于番茄HsfA3基因亚细胞定位(Subcellular location)和遗传转化的植物表达载体pBI-sl-A3、pBI-A3。将pBI121质粒上的35S启动子进行改造,分别构建了植物表达载体pBI-mini35S、pBI-HSE-mini35S,用于研究热激转录因子HsfA3与热激元件(Heat shockelements,HSEs)的互作。通过冻融法将以上4种重组质粒导入根癌农杆菌LBA4404中,用于后续试验,为进一步研究HsfA3基因在耐热中的功能和分子生物学机制奠定了基础。

Based on plasmid pBI121, recombinant plasmids pBI-sI-A3 and pBI-A3 driven by 35S promoter were constructed for subcellular location and transformation of tomato HsfA3 gene. After the improvement of 35S promoter on plasmid pBI121, another two recombinant plasmids pBI-mini35S and pBI-HSE-mini35S were obtained for the further study of the interaction between HsfA3 factor and HSEs. Then four recombinant plasmids were transformed into Agrobacterium LBA4404 by using freeze and thaw method, and these materials provided the basis for investigating the function and molecular mechanism of HsfA3 gene in heat tolerance.