类风湿关节炎患者外周血淋巴细胞趋化因子受体CCR7的表达及其临床意义

The Clinical Significance of CCR7 Expression on Lymphocyte in the Patients with Rheumatoid Arthritis

目的探讨CC族趋化因子受体CCR7在类风湿关节炎(RA)发病机制中的作用及其临床意义。方法选取30例RA患者(RA组)和20例健康体检者(正常对照组)。应用流式细胞检测术检测两组受检者外周血淋巴细胞表面CCR7的表达,并根据患者的触痛关节数、肿胀关节数、红细胞沉降率计算出患者的疾病活动性积分(DAS28),分析CCR7的表达与疾病活动性的关系。结果 RA组患者外周血CD4+T细胞和CD8+T细胞上CCR7的表达率〔(74.5±13.7)%与(57.9±11.8)%〕均显著高于正常对照组〔(16.7±2.0)%与(16.6±1.6)%〕,差异有统计学意义(P<0.01);且RA组患者外周血CD4+T细胞上CCR7的表达率显著高于CD8+T细胞,差异有统计学意义(P<0.05)。RA组患者外周血CD4+T细胞CCR7的表达与DAS28呈正相关(r=0.836,P<0.01)。结论 CCR7在RA患者外周血淋巴细胞上的表达异常增加,尤其是在CD4+T细胞上;CCR7在CD4+T细胞上的异常表达可能是导致RA病情活动的重要因素之一。

Objective To explore the role and the clinical significance of CCR7 in the pathogenesis of rheumatoid arthritis（RA）.Methods 30 patients with RA（RA group）and 20 healthy persons（normal control group） were selected.The expressions of CCR7 on the peripheral blood lymphocytes in the two groups were detected by flow cytometry（FCM）.Moreover,we calculated disease activity score（DAS28） of every patient with RA and analyzed the relativity of CCR7 expression and the disease activity.Results The levels of CCR7 in CD＋4T and CD＋8 T cells from RA group〔（74.5±13.7）% and（57.9±11.8）%〕were higher than that from normal controls〔（16.7±2.0）% and（16.6±1.6）%〕.The difference was significant（P0.01）.The level of CCR7 in CD＋4T cells from RA group was higher than that in CD＋8T cells,the difference was significant（P0.05）.The level of CCR7 in CD＋4 T cell was positively related to DAS28（r=0.836,P0.01）.Conclusion The abnormal expression of CCR7 is increased on peripheral blood lymphocytes from RA patients,especially on CD＋4 T cells.The abnormal expression of CCR7 on CD＋4T cells is probably one of the key factors resulting in the disease development of RA.