脓毒症诱导脑神经细胞凋亡的实验研究

Neuronal Apoptosis Induced by Sepsis

目的：研究脓毒症时脑神经细胞凋亡的发生情况及其发病机制。方法：采用胆管内注射大肠杆菌的方法制作大鼠急性重症胆管炎模型，４天后取血化验ＴＮＦα、ＩＬ－１及ＩＬ－６活性，并取脑海马组织作原位细胞凋亡检测及电镜检查。结果：（１）观察组比对照组血ＩＬ－１、ＩＬ－６及ＴＮＦα水平明显增高。（２）用ＴＵＮＥＬ染色法镜下观察发现，观察组的原位细胞凋亡数目较对照组增多。（３）电镜下观察发现海马区神经细胞发生早期细胞凋亡改变。结论：在大鼠发生急性重症胆管炎时，ＴＮＦα、ＩＬ－１及ＩＬ－６等细胞因子的明显增高为典型脓毒症表现，而后者可诱导脑神经细胞发生凋亡。

To investigate the mechanism of neuronal apoptosis during sepsis, the acute serious cholangitis rat models were established by means of injecting E-coli into the bile duct,and the normal animals were used as controls. All animals were killed after 4 days and their plasma were collected to determine the levels of TNF-α, IL-1 and IL-6. Furthermore, their brain tissues were collected to observe the neuronal apoptosis by TUNEL staining and electron microscopy. The results showed:The early neuronal apoptosis was found in the sepsis group by either staining or electron microscopy, and the levels of TNF-α, IL- 1 and IL-6 were higher than normal group. The study proved that the cytokines such as TNF-α, IL-1 and IL-6 were expressed highly in rats during acute serious cholangitis,sepsis can induce early neuronal aoptosis obviously.