小鼠15%Ⅲ度烧伤时骨髓基质细胞对粒单系祖细胞生长的影响

The Effect of Bone Marrow Stromal Cells of Mice with 15% TBSA Degree Burn on the Growth of Granulo-monopoietic Progenitors

目的研究烧伤情况下骨髓造血基质细胞支持粒单系造血的功能变化。方法采用体外小鼠骨髓基质细胞融合培养和粒单系祖细胞集落（ＣＦＵ－ＧＭ）培养的方法，动态观察１５％Ⅲ度烧伤时骨髓基质细胞对粒单系祖细胞生长的影响。结果当在烧伤组基质细胞层上培养正常骨髓细胞的ＣＦＵ－ＧＭ时，其集落生成能力在伤后１，３，５，７，１０天均明显低于正常对照组，而同时培养的烧伤小鼠骨髓细胞ＣＦＵ－ＧＭ集落则为伤后１～５天显著降低，第７天与正常组无差别，第１０天时明显超过正常，与正常基质细胞层上培养烧伤组ＣＦＵ－ＧＭ时有相同的变化趋势。结论烧伤时骨髓基质细胞支持粒单系造血的功能受到了一定程度的损伤，且较难恢复，而粒单系祖细胞的损伤则恢复较快。

Objective To investigate the effect of bone marrow stromal cells of mice with 15% TBSA degree burn on the growth of granulo-monopoietic progenitors. Methods The culture and confluent of the stromal cell of the mice bone marrow and semi-solid culture of the colony-forming unit of granulo-monopoietic progenitors (CFU-GM) in vitro were employed. Results The number of normal bone marrow CFU-GM on the surface of stromal cells layer in burned mice was significantly reduced as compared with controls at the days 1,3,5,7,10 post injury;whereas the burned mice bone marrow CFU-GM under the same cultural condition were also decreased at the days 1-5,but at days 7 and 10 the number was recovered and increased,respectively.The differences of the effect of bone marrow stromal cells and GM-CSF on the growth of CFU-GM were also investigated. Conclusions The supporting function of bone marrow stromal cells on CFU-GM is impaired under the condition of 15% TBSA degree burn in mice.