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从微量全血直接进行HLA-DRB1基因配型的技术及其应用 被引量：2

Trace blood HLADRB1 typing by means of PCRSSP
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摘要 目的适应临床器官基因配型的需要。方法建立从微量全血不经抽提ＤＮＡ，直接进行ＰＣＲＳＳＰ完成ＨＬＡＤＲＢ１位点基因分型，结果与血清学分型方法对照。部分分型结果用直接测序法（ＳＢＴ）验证。结果５０例标本ＤＲＢ１配型２２例与血清学分型结果一致。对于结果不一致者，采用ＳＢＴ分型技术进行验证，其分型结果与用微量全血直接进行ＰＣＲＳＳＰ的分型结果一致。结论从微量全血直接进行ＨＬＡＤＲＢ１基因配型的技术准确可靠。 Objective To establish a convenient and practical trace blood DNA typing technique. Methods Trace blood without DNA extraction was used for DNA typing by means of PCRSSP and the results were checked by sequencebasedtyping (SBT) method. Results 50 samples of trace blood were successfully typed by means of PCRSSP and the procedure takes about 2 h.The accordance rate between the trace blood PCRSSP and the serology method has been 68.8%. Conclusions5BZThe trace blood PCRSSP typeing technique is reliable and accurate.
作者 张晶 蒋宇光 朱章菱 许建军 马潞林 周艳 高居忠 刘敬忠
机构地区 首都医科大学附属北京红十字朝阳医院
出处 《中华泌尿外科杂志》 CAS CSCD 北大核心 1999年第6期356-358,共3页 Chinese Journal of Urology
关键词 肾移植 基因型 HLA-DRB1 基因配型 KidneyTransplantationGenotype
分类号 R699.2 [医药卫生—泌尿科学]
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参考文献3

共引文献17

同被引文献26

  • 1曹盂德 秦东春 孙含笑.HLA分子生物学及临床应用[M].河南:河南医科大学出版社,1998.280-284.
  • 2Suberbielle-Boissel C, Chapuis E, Charron D, et al. Comparative study of two methods of HLA-DR typing: serology and PCR/Dot blot reverse. Transplant Proc, 1997, 29:2335-2336.
  • 3Bunce M, O' Neill CM, Barnardo MC, et al. Phototyping:comprehensive DNA typing for HLA-A, -B, -C, DRBI, DRB3, DRB4,DRBS&DQBI by PCR with 144primer mixes utilizing sequence-specific primers(PCR-SSP). Tissue Antigens, 1995,46:355-367.
  • 4Chen DF, Seibert I, Chen HY, et al. Improvement of HLA class I and class II PCR-SSP typing by using timed-release activity of DNA polymerase. Tissue Antigens, 1998, 51:645-648.
  • 5Hein J, Bottcher K, Grundmann R, et al. Low resolution DNA typing of the HLA-B5 cross -reactive group by nested PCR-SSP.Tissue Antigens, 1995,45:27-35.
  • 6Carter AS, Cerundolo L, Bunce M, eL al. Nested polymerase chain reaction with sequence -specific primers typing for HLA-A,-B, and -C alleles:detection of microchimerism in DR-matched individuals.Blood, 1999, 94:1471-1477.
  • 7Olerup O, Zetterquist H. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens, 1992, 39 :225-235.
  • 8Drabek J, Vranova K, Ambruzova Z, et al. Class I typing by PCR-SSP in Olomouc. Bone Marrow Transplant 1998,4(Suppl) :S23-26.
  • 9Mytilineos J, Lempert M, Scherer S. et al. Comparison of serological and DNA PCR-SSP typing results for HLA-A and HLA-B in 421 black individuals:a Collaborative Transplant Study report.Hum Immunol ,1998, 59:512-517.
  • 10Emonds MP, Mytilineos Y, Scherer S, et al. A single center evaluation of the collaborative transplant study (CTS) DNA project.Transpl Int ,1996,9:468-475.

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中华泌尿外科杂志
1999年 第6期

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