人正常尿路移行上皮细胞无血清培养

Culture of human normal urothelial cells with serumfree medium

目的建立一种比较简便的人正常尿路上皮细胞无血清培养方法。方法标本组织块用冷消化法分离出尿路上皮细胞，培养于无血清生长培养基。观察传代细胞形态及生长情况，并用ＴＧＦβ１抑制细胞增殖试验检验细胞在有血清或无血清培养基中对ＴＧＦβ１的反应。结果培养的总成功率为７３％，细胞具有典型的移行上皮细胞形态学特征，细胞能在２４小时后增殖进入对数生长期，传代９～１１次后开始衰退。另外，无血清培养体系比含血清培养体系更能反映出ＴＧＦβ１对细胞抑制作用。结论此培养体系具有简单、短期内可扩增出大量纯净上皮细胞、应用范围广和培养成功率高的特点。

Objective To establish a relative simple and convenient serumfree culture method of human unthelial cells. Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serumfree growth medium.Growth and morphology of passaged cells were observed.Moreover,cells reacting to TGF1 was cheked with TGF1 inhibiting cell growth test in serum-free or containing serum medium. Results Total successful rate of culture was 73%.Cultured cells had typical morphological features of transitional epithelium and rapidly proliferated into logarithm growth period.After 911 passages,cells began senescence.The changes of cell proliferation in serumfree medium rather than serum containing medium reflected the TGF1 effect. Conclusions This culture system was simple and convenient,amplifying a lot of pure epithelial cells in short term with more applicability and high successful culture rate.