摘要
目的:建立淡竹叶中日当药黄素的含量测定方法。方法:采用HPLC法测定不同产地、不同采收期淡竹叶中日当药黄素的含量。结果:日当药黄素在0.672-4.704μg范围内线性关系良好;平均加样回收率在93.9%-104.0%之间,RSD为100.92%。结论:该方法操作简便,重现性高,特异性强,能准确的测定淡竹叶中日当药黄素的含量,为评价淡竹叶的质量提供了有效方法。
Objective: To develop a method for determination of swertiajaponin in Lophatherum gracile Brongn.Method: The HPLC method was applied and determined swertiajaponin in Herba Lophatheri from different habitats and harvesting time.Result: In the range of 0.672~4.704μg,the linear relationship were excellent;the mean recoveries were between 93.9% and 104.0%,and RSD values were 100.92%.Conclusion: The method is simple,high reproducibility,specificity,can determinate the swertiajaponin in Herba Lophatheri accurately,which provide an effective method to evaluate the quality of Herba Lophatheri.
出处
《中医药信息》
2010年第5期11-13,共3页
Information on Traditional Chinese Medicine
基金
南京中医药大学科技创新团队项目(2007)
江苏省教育厅高新技术项目基金(JHB04-039)
江苏高校科研成果产业化推进项目(JHZD09-11)
关键词
淡竹叶
日当药黄素
含量测定
Herba Lophatheri
swertiajaponin
determination