Nup88在结直肠癌组织中的表达及意义

Expression and significance of Nup88 mRNA in colorectal cancer

目的研究核孔蛋白复合体蛋白88(Nup88)蛋白和mRNA在结直肠癌组织中的表达及其与临床病理特征的关系。方法免疫组化方法检测181例结直肠癌标本、18例淋巴结转移癌及84例匹配的癌旁无瘤黏膜组织中Nup88蛋白表达;RT-PCR法检测Nup88 mRNA在29例结直肠癌标本及配对的癌旁2 cm组织、切缘黏膜中的表达水平。结果结直肠癌组织和淋巴结转移癌中Nup88蛋白阳性率均高于癌旁无瘤黏膜组织(P<0.01);Nup88蛋白的表达与结直肠癌组织分化程度有关。Nup88 mRNA在肿瘤组织中的表达水平高于癌旁无瘤黏膜和切缘黏膜(P均<0.01)。结论 Nup88蛋白及mRNA与结直肠癌的发生发展有关,其蛋白表达可作为评估结直肠癌生物学行为的指标之一。

Objective To investigate the expression of Nup88 protein and mRNA in colorectal cancer as well as their relationship with clinicopathological features.Methods Nup88 protein expression was examined by using histochemistry in 181 cases of colorectal cancer,18 cases of lymph node metastasis carcinoma and 84 matched cases of non-carcinoma mucosa.Nup88 mRNA expression was examined by using RT-PCR in 29 colorectal cancer,matched cases of mucosa of surgical margins and non-carcinoma mucosa which were 2 cm away from tumors.Results The positive rate of Nup88 protein expression in colorectal cancer and lymph node metastatic carcinoma were both significantly higher than that in non-carcinoma mucosa（P0.01）.The expression of Nup88 protein in colorectal cancer was related with tissue differentiation degree.Nup88 mRNA expression was higher in colorectal cancer than that in non-carcinoma mucosa and mucosa of surgical margins（P0.01）.Conclusions Nup88 plays a role during the development of colorectal cancer.Nup88 protein may be an indicator to evaluate the biological behaviour of colorectal cancer.