Bcl-2、P53、CyclinD1在B细胞淋巴瘤中的表达及意义

Expression and clinical significance of Bcl-2,P53,CyclinD1 in B-cell lymphoma

目的探讨Bc l-2、P53和细胞周期蛋白D1(CyclinD1)在B细胞淋巴瘤发生发展中的作用。方法采用组织芯片技术制备高通量的样本,采用免疫组织化学SP法检测Bc l-2、P53、Cyc linD1在B细胞淋巴瘤中的表达。结果 Bc l-2和P53主要表达于弥漫性大B细胞淋巴瘤、滤泡性淋巴瘤、黏膜相关淋巴组织结外边缘淋巴瘤、套细胞淋巴瘤和小淋巴细胞淋巴瘤;而CyclinD1只在套细胞淋巴瘤和弥漫性大B细胞淋巴瘤中表达。结论由Bc l-2和P53协同表达途径以及CyclinD1异常表达途径是弥漫性大B细胞淋巴瘤发生的重要机制。

Objective To investigate the expression of Bcl-2,P53,CyclinD1 in B-cell lymphoma and their relevance with the development of B-cell lymphoma.Methods The preparation of high-throughput sample was made by method of tissue microarray.The expression of Bcl-2,P53,CyclinD1 in B-cell lymphoma was examined by using immunohistochemical staining.Results The expression of Bcl-2 and P53 was significantly higher in DLBCL,FL,MALT,MCL and SLL than that in normal tissues.CyclinD1 was only detected in DLBCL and MCL.Conclusions The coordination expression of Bcl-2 and P53 and the abnormal expression of CyclinD1 were two important mechanism of the occurrence of DLBCL.