海藻酸钠纯度对大鼠微囊纤维化和囊内肝细胞功能的影响

The effect of alginate purity on microencapsule overgrowth and function of microencapsulated hepatocyte in peritoneal transplantation

目的研究海藻酸钠纯度对微囊纤维化及囊内肝细胞组织形态、功能的影响。方法采用逐步过滤和氯仿和丁醇萃取蛋白的方法纯化海藻酸钠，用纯化和未纯化的海藻酸钠制作两种微囊化肝细胞，分别作同种异体腹腔移植并观察移植后不同时期（２周、１、２、和６个月）微囊和囊内肝细胞的组织结构、功能表达及对急性肝衰大鼠存活率的影响。结果纯化组的微囊回收率较未纯化组明显增高（Ｐ＜０．００１）。纯化组移植１个月时大部分肝细胞组织形态、酶和白蛋白染色与正常肝细胞差异不显著，未纯化组移植１个月内肝细胞破坏。长期移植（２、６个月），纯化组微囊仍保持完整光滑，但肝细胞破坏；纯化组的微囊化肝细胞治疗急性肝衰较未纯化组效果明显。结论纯化海藻酸钠能降低微囊的纤维化程度，改善囊内肝细胞功能。

Objective To study the effect of alginate purity on microencapsule overgrowth, histology and function of microencapsulated hepatocyte (MH). Methods Alginate was purified by filtration and chloroform/butanol extraction. Using purified and crude alginate to make two kinds of MHs, we transplanted each kind of MHs into the peritoneal cavities of rats. After 2 weeks, 1, 2 and 6 months, MHs were washed out to study overgrowth of MH, histology, and expression of enzyme and albumin in hepatocytes. Two kinds of MHs (the purified and the crude) were transplanted to FHF rats separately to study their differences in liver function support. Results The amount of retrieved MHs was larger in the purified than in the crude ( P <0.001). In the purified group, most hepatocytes had normal morphology, positive enzymatic histochemistry and albumin immuno histochemistry stain in one month of transplantation but were damaged in the crude group only 2 weeks later. Microencapsules were smooth and no overgrowth was observed during 6 months in the purified but all overgrowth one month later after transplantation in the crude. The purified MHs had excellent liver function support in the FHF comparing with the crude and the control. Conclusion The purified alginate may significantly reduce the overgrowth of MHs and improve the function of hepatocytes.