Vc/FeSO_4诱发红细胞膜脂质过氧化模型的建立

The Foundation of Erythrocyte Membranes Lipid Peroxidation Model Induced by VC/FeSO 4

通过体外实验，对Ｖｃ／ＦｅＳＯ４诱发的红细胞膜脂质过氧化（ＬＰ）发生条件及反应历程进行了探讨。结果表明：在３７℃水浴４５分钟内，红细胞膜蛋白浓度为０．７５ｍｇ／ｍｌ，５．０μｍｏｌ／ＬＦｅＳＯ４，ｐＨ值７．４的５ｍｍｏｌ／Ｌ磷酸盐缓冲液的条件下，较低浓度Ｖｃ（小于１．０ｍｍｏｌ／Ｌ）时可促进红细胞膜脂质过氧化过程，但高浓度Ｖｃ（大于１．０ｍｍｏｌ／Ｌ）则抑制红细胞膜脂质过氧化过程。Ｖｃ浓度为０．２５ｍｍｏｌ／Ｌ时，红细胞膜脂质过氧化产物丙二醛（ＭＤＡ）于反应后４５分钟内迅速上升，９０分钟接近峰值，以后趋于平衡。揭示：一定比例的Ｖｃ／ＦｅＳＯ４可有效地激发红细胞膜ＬＰ。本研究初步建立了红细胞膜ＬＰ模型。

The condition and the time course of lipid peroxidation(LP)induced by VC/FeSO 4 in human erytrocyte membranes in vitro was studied.The results revealed that,when human erythoccyte membranes(0 75mg/ml protein)were incubated with 5μmol/L PBS buffer for 45 minutes,VC,at low concentration(<1 0 mmol/L)stimulate lP,but at high concenration(>1 ) mmol/L),inhibated LP.The content of malonaldehyde(MDA)rised rapidly after additon of 0 25 mmol/L VC and 5 0μmol/L FeSO 4,reached maxinal level in 90 minutes,very little additional LP occured over the next two hours.The result suggested that in 45 minutes,VC/FeSO 4 the appropriate scale could induced effectively erythrocyte membranes LP.A model induced by VC/FeSO 4 was founded preliminarily in our experimnet.