马铃薯种质资源保存试验

Preservation of Potato Germplasm

通过低温保存,添加渗透调节剂及生长抑制剂和微型薯诱导的方法,对马铃薯种质资源试管苗的保存效果进行了研究。结果表明,使用低温(4℃)保存和在MS培养基基础上添加浓度为50mg·L-1的生长抑制剂比久(B)9,可以使试管苗保存时间长达8～10个月,且苗茎叶健壮,有试管薯形成,保存效果较好;MS基础上添加浓度为0.01%～0.1%渗透调节剂甘露醇也能达到较好的保存效果;通过微型薯诱导保存得到试管薯的诱导率为15%～80%,但保存后期易造成试管苗死亡。

The way of potato germplasm preservation was investigated,including low temperature,addition of permeable regulator and growth inhibitor,and induction of microtuber.The low temperature preservation（4℃） and adding the growth inhibitor B9 at the concentration of 50 mg·L-1 to MS culture medium could preserve potato plantlets in vitro for up to 8-10 months,under which conditions the plantlets in vitro were strong and produced some microtubers,and therefore,better results were achieved.Addition of 0.01% -0.1% penetration regulator mannitol to basal MS medium could also achieve similar results.When cultured in various inducing media,the plantlets in vitro produced microtubers at the rate of 15%-80%,however,it gave rise to the death in the late stage of preservation.