马铃薯环腐病菌Real-time Taqman-PCR检测体系的建立

Establishment of Fluorescent Quantitation PCR Detection System(Taqman) for Clavibacer michiganensis subsp.sepedonicus

本研究根据环腐病菌16S rRNA保守序列设计特异性引物及探针,建立了马铃薯环腐病菌实时定量荧光PCR检测体系。研究表明,该体系可以准确、稳定、定量的检测出样品中最低浓度为1fg·μL-1(即3.21×103copies·μL-)1的目的基因,检测极限可以达到几个拷贝。该检测体系可对马铃薯环腐病菌进行微量检测,对于保证马铃薯各级种薯、商品薯及其相关产品的质量,提高市场竞争力具有重要意义。

According to 16 S rRNA conserved sequence of Clavibacer michiganensis subsp.sepedonicus,specific primers were designed,and fluorescent quantitation PCR detection system（Taqman） of potato ring rot was established.In the case of definite quantitative determination,the testing capability was 1 fg·μL-1（3.21 ×103 copies·μL-1） for real-time quantitative PCR（Taqman）.It could detect several copies of genes by fluorescent quantitation PCR detection system（Taqman）.The detection system can be established for trace detection of potato ring rot,ensuring the quality of seed potatoes,ware potatoes,and related potato products,thereby improving the market competitiveness.