高速逆流色谱法分离制备抱茎獐牙菜中龙胆苦苷和獐牙菜苦苷

Separation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H. Smith by High Speed Counter-current Chromatography

[目的]建立抱茎獐牙菜(Swertia franchetiana H.Smith)中分离制备龙胆苦苷和獐牙菜苦苷的高速逆流色谱法。[方法]采用溶剂系统为氯仿∶甲醇∶水=4∶4∶2(V/V/V);上相为固定相,下相为流动相;上下相超声波脱气20min;转速850r/min;流动相流速2ml/min;检测波长254nm。所得产物用旋光仪测定其旋光度,计算其比旋光度,进行纯度鉴定。[结果]从100mg抱茎獐牙菜粗提物中得到龙胆苦苷14mg,獐牙菜苦苷27mg。测得龙胆苦苷和獐牙菜苦苷的比旋光度[a]Dt分别为-196.3°和-126.6°,纯度较高。[结论]该研究可为用高速逆流色谱法大量制备龙胆苦苷和獐牙菜苦苷提供可行的方法。

[Objective]The aim was to establish the high speed counter-current chromatography （HSCCC） to successfully separate and prepare gentiopicroside and sweroside from the extracts of Swertia franchetiana H.Smith.[Method] A two-phase solvent system composed of chloroform：methanol∶water=4∶4∶2 （V/V/V） was used; the upper phase was stationary phase while the lower was mobile phase; they were pumped into the multilayer-coiled column simultaneously by using an ultrasonic degassing for 20 min; the rotating velocity is 850 r/min; the lower phase was pumped at a flow rate of 2 ml/min; and the detected wavelength is 254 nm.[Result]14 mg of gentiopricroside and 27 mg swersoside were obtained from 100 mg of the crude extracts.And it is detected that the specific rotation[a]Dt of gentiopicroside and sweroside were-196.3° and-126.6° respectively,the purity is high.[Conclusion]The study provides a feasible way to prepare a large number of gentiopicroside and sweroside by high speed counter-current chromatography.