3种苜蓿不同部位皂苷含量的测定研究

Determination of Saponin Content in Different Parts of 3 Kinds of Alfalfa

[目的]研究3种苜蓿不同部位中皂苷含量的测定。[方法]采用比色法测定了江苏地区苜蓿属3种植物紫花苜蓿(包括5种栽培品种)、天蓝苜蓿和南苜蓿不同部位的总皂苷含量。[结果]紫花苜蓿根中皂苷含量(1.39%)明显高于其他2种苜蓿根中皂苷含量;天蓝苜蓿和南苜蓿茎中皂苷含量差异不明显(RSD=2.4%),略低于紫花苜蓿茎中皂苷含量(0.583%);天蓝苜蓿叶中皂苷含量最低(0.623%)。综合3种不同部位的皂苷含量,根中皂苷的平均含量达到1.335%,明显大于茎(0.596%)和叶(0.655%)中皂苷含量。[结论]该研究为进一步开发苜蓿属植物资源提供了一定的理论依据。

[Objective]The aim was to studt the determination of saponin content in different parts of 3 kinds of alfalfa.[Method]Contents of total saponin in different parts of Medicago sativa L.（including 5 cultivars） ,Medicago lupulina L.and Medicago polymorpha L.from Jiangsu Province were determined by colorimetric method.[Result] Saponin content （1.39% ） in the root of Medicago sativa L.was obviously higher than those in Medicago lupulina L.and Medicago polymorpha L.; There was no obvious difference between saponin contents in the stems of Medicago lupulina L.and Medicago polymorpha L.（RSD=2.4% ） ,which were slightly lower than that of Medicago sativa L.（0.583%） ; The leaf of Medicago lupulina L.had the lowest saponin content （0.623%） .Combining the saponin contents in the 3 different parts,it was found that average content of saponin in root reached 1.335% ,which was obviously more than those in stem （0.596% ） and leaf （0.655%） .[Conclusion]The research provides a certain theoretical basis for the further exploitation of Medicago Linn.resources.