改构型酸性成纤维细胞生长因子对紫杉醇诱导的人胚肝细胞L-O2及人胚肾细胞293损伤的保护作用

Protective Effect of aFGF on Paclitaxel-induced Nephrotoxicity in Human Liver Cell L-O2 and Human Kidney Cell 293 in Vitro

目的观察紫杉醇对人胚肝细胞L-O2及人胚肾细胞293的毒性作用,及加入改构型酸性成纤维细胞生长因子(aFGF)后对紫杉醇引起的肝肾细胞损害的保护,并探讨其可能机制。方法用紫杉醇诱导人胚肝细胞L-O2及人胚肾细胞293损伤,同时加入aFGF对肝、肾细胞进行保护,并测定培养上清液中的SOD,MDA,LDH值,MTT法测定肝、肾细胞活性。结果紫杉醇在体外对人胚肝细胞L-O2、人胚肾细胞293有一定的毒性作用,使用aFGF对肝、肾细胞进行保护后,IC50值有明显的提高,SOD值与未加aFGF组相比明显升高,而MDA,LDH值明显降低(P<0.05)。结论 aFGF可有效对抗紫杉醇引起的肝、肾细胞损伤。

Objective To investigate the protective effects of aFGF on paclitaxel-induced nephrotoxicity in human liver cell L -O2 and human kidney cell 293 and its possible mechanism. Methods The effect of aFGF on paclitaxel-induced nephrotoxicity was evaluated by MTT assay. The contents of SOD,MDA and LDH in cultural supernatant were determined by general methods. Results Paclitaxel could inhibit the proliferation of human liver cell L -O2 and human kidney cell 293 in a dose-dependent manner. aFGF could protect human liver cell L-O2 and human kidney cell 293 injury induced by paclitaxel（ P 0. 05） . Conclusion Paclitaxel has the function of inhibiting proliferation on human liver cell L -02 and aFGF can exert obvious protective effects on the cells.