自然流产患者蜕膜组织疾病特异蛋白的初步筛选与鉴定

Preliminary screening and identification of spontaneous abortion decidual tissue special protein

目的建立早期自然流产蜕膜组织和早期正常蜕膜组织双向电泳图谱,并观察两者之间蛋白表达的差异。方法采用双向电泳技术分离蜕膜组织蛋白,利用专用软件分析所得差异明显蛋白并使用免疫组化和Western blotting验证双向电泳结果。结果成功建立了早期自然流产蜕膜组织和早期正常蜕膜组织双向电泳图谱,并鉴定出9个差异显著的蛋白。结论 9个差异显著的蛋白的功能主要涉及绒毛外滋养细胞的侵袭、自然流产蜕膜组织血管的重建以及蜕膜细胞的凋亡等方面,为进一步研究自然流产发病机制和治疗手段奠定了基础。

Objective To investigate the differential proteomic expressions between the early spontaneous abortion decidual tissue and its normal control.Methods Samples of decidual protein were separated by two-dimensional gel electrohporesis（2-DE）.The screened differentially protein expressed protein spots were identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS）,and key of which was verificated by Western blotting and immunohistochemical staining.Results The two dimensional polyacrylamide gel electrohporesis profiles of decidual tissue protein were successfully established by 2-DE.According to MALDI-TOF-MS,9 proteins were significant.Conclusion These differential expression proteins mainly refer to invasion of the blastocyst,blood vessel remodeling and cell apoptosis.This can be an experimental basis for further research of the pathogenesis and treatment of spontaneous abortion.