S-TE预处理污泥厌氧发酵产氢

BIO-HYDROGEN PRODUCTION FROM S-TE PRETREATED SLUDGE BY ANAEROBIC FERMENTATION

应用嗜热酶污泥溶解(S-TE)技术预处理剩余污泥,研究接种外在产氢菌(Enterococcus sp.LG1)和未接种外在产氢菌两种状况下,污泥发酵的产氢效果,并与相应温度(65℃)热预处理污泥的发酵产氢效果进行对比,分析探讨了污泥发酵产氢过程中底物和pH值的变化。结果表明:经S-TE预处理的污泥在未接种外在产氢菌时,产氢效果良好,最大产氢率(H_2/VS)高达16.3mL H_2/g,高出65℃热预处理污泥接种产氢菌15.6%,高出65℃热预处理污泥未接种产氢菌26.4%,发酵气体中只含有H_2和CO_2,不含CH_4,氢延迟时间短(3～4h),产氢率达最大值后能较稳定维持10h以上;S-TE预处理污泥接种产氢菌后,产氢效果不佳,最大产氢率仅为10.7mL/g。S-TE预处理污泥发酵过程中,可溶性蛋白质和可溶性糖是产氢发酵的主要营养物质。

Batch tests were carried out to analyze the effects of anaerobic fermentative hydrogen production using excess sludge pretreated by S-TE （ solubilization by thermophilic enzyme） with and without inoculation of hydrogen-producing bacteria, Enterococcus sp. IG1. The performance of biohydrogen production of the S-TE pretreated sludge and the 65 ~C thermally pretreated sludge was also compared in batch fermentation tests. The changes of sludge substrates and pH value during the fermentation process were also monitored and discussed. The results showed that sludge pretreated by S-TE without inoculation could make good bio-hydrogen production. The maximal hydrogen yield （H2/VS） was 16.3mL H2/g, 26.4% higher than 65℃ thermally sludge pretreated without inoculation and 15.6% higher with inoculation. Hydrogen and carbon dioxide were only produced and methane was not observed during the process. The lay time for hydrogen pro- duction was only 3-4 hours, the bio-hydrogen was able to maintain stable for above 10 hours with little consuming after reaching its maximum. The bio-hydrogen yield of the S-TE pretreated sludge inoculated Enterococcus sp. LG1 was very low, only 10.7mL/g. Soluble protein and carbohydrate were the main substrates for bio-hydrogen fermentation from S-TE pretreated sludge.