塑料增塑剂DEHP环境污染水平、人体负荷及生物毒性的初步研究

Study on exposure level in environment media,human body loading and toxic effects of Di(2-ethylhexy1) phthalate

目的为了初步研究邻苯二甲酸二乙基己酯(Di(2-ethylhexy1)phthalate,DEHP)的环境污染水平,采用气相色谱技术检测常用塑料容器浸提液中DEHP的含量,部分样品中检测出DEHP。方法为了初步调查DE-HP在人体中的负荷水平,运用高效液相色谱技术检测中学生尿液中DEHP主要代谢产物邻苯二甲酸单乙基己酯(MEHP)的含量,其检出率达到60%。结果为了进一步探讨DEHP的生物毒性作用,以大鼠肝脏、肾脏细胞作为受试对象,以0、5、20、80μmol.L-1的DEHP溶液对其体外染毒1h,用丙二醛(malondia ldehyde,MDA)含量和DNA-蛋白质交联(DPC)系数的变化为测试指标。结论结果表明,随着DEHP浓度的升高,大鼠肝脏、肾脏细胞的MDA含量和DPC系数均上升,说明DEHP能够通过氧化损伤途径对大鼠肝脏、肾脏细胞造成损伤。

Objective To explore the exposure level in environment media,the concentration of DEHP in plastic containers was studied by gas chromatography.Methods The concentration of MEHP（the main breakdown chemical of DEHP） in students＇ urine was measured by high performance liquid chromatography.ResultsFurthermore,the toxic effect of DEHP in liver,kidney cells of rat was also observed.The exposure concentration of DEHP was 0,5,20 and 80 μmol·L^-1.Conclusions After 1-hour exposure,the content of malondialdehyde（MDA） and the coefficient of DNA-protein crosslink（DPC） were examined.Results showed both MDA content and DPC coefficient in liver and kidney cells increased gradually with the increase of DEHP concentration It was suggested that DEHP could induce acute oxidative damage in liver and kidney cells of rat.