胚胎干细胞标志CD31、SSEA-1在小鼠胚胎发育和成体组织中表达的初步研究

Primary Study of the Expression of Embryonic Stem Cell Markers CD31 and SSEA-1 in Developing Embryos and Adult Tissues

目的观察胚胎干细胞表面标志CD31、SSEA-1在小鼠胚胎发育过程中不同阶段的表达及在新生鼠、成体骨髓、睾丸中的表达情况,为进一步利用它们作为分子标志从胚胎及成体组织中分离多潜能性干细胞奠定基础。方法本实验选用129/SV系小鼠。孕10.5天、14.5天、18.5天的胚胎各取3例,新生鼠、6周龄成年鼠骨髓、成体睾丸各取3例,0.3%胶原酶消化获得单细胞悬液,经抗体染色标记后,通过流式细胞仪分析CD31、SSEA-1的表达情况。结果胚胎第10.5天、14.5天、18.5天和新生鼠中均有CD31、SSEA-1的表达,阳性细胞量随胚胎发育呈下降趋势。成年鼠骨髓和睾丸组织中也有CD31、SSEA-1阳性细胞存在,含量低于胚胎中的数量。结论 CD31、SSEA-1在小鼠个体发育过程以及成体组织中都有不同程度的表达,阳性细胞比率随个体发育而降低。

Objective To detect the expression of CD31 and SSEA-1 at different stages of developing embryos,as well as their expressions in neonates,adult bone marrow and testis.Methods 129/SV mice were mated.Embryos at 10.5 postcoitus（E10.5）,14.5 and 18.5 days were collected.In addition,neonatal mice,bone marrow and testis from 6-week-old mice were also collected.Single cell suspension was achieved by digestion with collagenase.After anti-CD31 and anti-SSEA-1 antibody labeling,cells were analyzed by flow cytometry.Results CD31 and SSEA-1 were expressed in E10.5,E14.5 and E18.5.The expression levels were decreased with the development of embryos.Meanwhile,both markers were observed in neonates,adult bone marrow and testis,but the level was lower than in embryos.Conclusion A certain amount of CD31 and SSEA-1 expressing cells are existing in developing embryos as well as in adult tissues.