IFN-γ上调MHC-Ⅱ分子表达对KC免疫原性的影响

The Effect of IFN-γ Increasing MHC-Ⅱ Expressing to the Immunogenicity of Keratinocytes

目的观察在炎症因子IFN-γ的作用下,角质形成细胞(Keratinocyte,KC)免疫原性的变化情况。方法 KC体外传代培养,按不同的INF-γ浓度分组后作用于传代的KC。将高表达MHC-Ⅱ分子的KC与异体淋巴细胞混合培养后,观察淋巴细胞的增殖情况;同时,将KC植入异体小鼠皮下,观察免疫排斥迹象。结果在6 000 U/mL IFN-γ的作用下,MHC-Ⅱ+的KC超过90%。高表达MHC-Ⅱ分子的传代KC无明显刺激异体淋巴细胞增殖的作用,植入异体小鼠皮下后也无明显淋巴细胞浸润现象。结论传代后的KC在IFN-γ作用下高表达MHC-Ⅱ分子,表达MHC-Ⅱ分子的KC无明显免疫原性。

Objective To observe changes of immunogenicity of keratinocytes（KC） under the influence of interferon-γ（IFN-γ）.Methods KC were cultured and passaged in vitro.Different dose of IFN-γ stimulated KC to express MHC-Ⅱ molecules.KC with highty expressed MHC-Ⅱ molecules were mixed with allogenic lymphocytes to observe their proliferation.Simultaneously,MHC-Ⅱ＋ KC were implanted subcutaneously into allogenic mice to observe the sign of immunological rejection.Results Under the influence of 6 000 U/mL IFN-γ,the percentage of MHC-Ⅱ＋ KC exceeded 90%.MHC-Ⅱ molecules highly expressed KC had no effects to stimulate proliferation of allogenic lymphocytes significantly.There were no obvious lymphocytes infiltration when MHC-Ⅱ molecules highly expressed KC were implanted subcutaneously into allogenic mice.Conclusion Passaged KC could express MHC-Ⅱ molecules highty in the influence of IFN-γ,KC expressed MHC-Ⅱ molecules has no obvious immunogenicity.