利用木糖生产中的纤维废弃物制备功能性纤维低聚糖的研究

Study of Functional Cello-Oligosaccharide Preparation from the Celluosic Waste of Xylose Manufacture

以木糖制取工业的纤维废弃物木糖渣(100g·L-1)为原料,利用纤维素酶系不同组分间的协同作用,定向降解制备功能性纤维低聚糖,并对该工艺过程的关键参数进行了考察。结果表明:采用不溶性碳源发酵生成的纤维素酶,其CBA/FPA比例较低(<0.02),适宜于制备纤维低聚糖;酶用量选用10～15FPIU·g-1底物;酶解时间控制在8～24h内,通过严格调节反应时间可获得不同聚合度的纤维低聚糖产品;在反应过程中适时移去酶解液,可有效分离去除纤维二糖酶,并利用吸附在固体残渣上的C1酶和Cx酶重复进行四批酶解反应,纤维低聚糖的得率均保持在70%以上。研究结果提供了一条经济高效制备纤维低聚糖的工艺路线,对于促进可再生纤维素资源的转化利用具有重要意义。

Using the cellulosic waste corncob residue from xylose manufactory as substrate（100 g·L-1）,the functional cello-oligosaccharide was prepared specifically by synergetic hydrolysis of cellulase.The main parameters of the preparation were investigated,and the results show that the cellulase induced by insoluble carbon source,which has low ratio of CBA to FPA（0.02）,is suitable for cello-oligosaccharide preparation.The optimal cellulase dosage for cello-oligosaccharide preparation is 10～15 FPIU·g-1 substrate.The feasible hydrolysis time is 8～24 h,and by accurate regulation of reaction time,cello-oligosaccharide with different polymerization degrees can be obtained.During the hydrolysis process,cellobiase can be separated by removal of hydrolysate,and enzymes of C1 and CX adsorbed on substrate residue can be reused for four times,with which the yields of cello-oligosaccharide keep above 70%.The research results provide an effective and economic technique for cello-oligosaccharide production,and are of benefit to accelerate the bioconversion of renewable cellulosic resources.