共刺激分子CD40在结肠癌中的表达及其临床意义

The Expression of the Costimulatory Molecule CD40 in Colon Cancer and Its Clinical Significance

目的:分析共刺激分子CD40在结肠癌中的表达及其与结肠癌临床病理特征的关系,探讨CD40在结肠癌发病中的作用。方法:应用免疫组织化学方法检测40例结肠癌组织及癌旁组织和30例正常结肠组织中CD40的表达,并分析其不同表达与患者年龄、性别、肿瘤大小、组织病理分级、TNM分期和淋巴结转移等临床病理特征的关系。同时采用直接免疫荧光标记和流式细胞仪检测CD40分子在结肠癌细胞株的表达。结果:40例结肠癌组织中CD40的表达率为67.5%,显著高于结肠癌旁组织和正常结肠组织(P<0.05)。直接免疫荧光标记和流式细胞仪检测结肠癌细胞株HCT116和SW48中CD40阳性表达率分别为45.8%和30.2%。结肠癌组织中CD40的高表达与肿瘤TNM分期和淋巴结转移相关(P<0.05)。结论:CD40在结肠癌组织中有较高的表达率,在促进结肠癌的侵袭转移中可能起重要作用,可望作为判断结肠癌预后及监测淋巴结转移的指标。

Objective：To investigate the expression of costimulatory molecule CD40 in colon cancer and its relationship to the clinicopathological features of colon cancer.Methods： Expression of CD40 was evaluated by immunohistochemical study in 40 colon cancer specimens,40 paracancerous specimens and 30 normal colon specimens.The relation between the staining expression and clinicopathological features including age,sex,tumor size,pathological grade,TNM stage and lymph node metastasis was examined.Colon cancer cell 1ines HCT116 and SW48 were also examined by direct immunofluorescence staining and flow cytometry.Results： The high expression rate of CD40 in 40 colon cancer specimens was 67.5%.The lever of CD40 expression was significantly higher in colon cancer than in normal colon and paracancerous tissues（P0.05）.The expression rates of CD40 in 2 colon cancer cells cel1 1ines were 45.8% and 30.2%,respectively.High expression of CD40 was correlated significantly with TNM stage and lymph node metastasis（P0.05）.Conclusions： CD40 is expressed in colon cancer at a high frequency,which may play an important role in invasion and metastasis of colon cancer and may be a valuable marker in predicting the prognosis of colon cancer.