肿瘤病人皮肤成纤维细胞离体培养及放射敏感性测定

CULTURE OF CANCER PATIENT'S SKIN TISSURE FIBROBLAST AND RADIOSENSITIVITY ASSAY

试图通过对肿瘤病人皮肤成纤维细胞的离体培养和集落形成实验，检测正常皮肤组织的放射敏感性，为探索正常组织的辐射耐受性提供理论依据。运用组织贴块法体外培养肿瘤病人皮肤成纤维细胞；采用１３７Ｃｓγ射线，不同剂量照射，以克隆形成法测定细胞的放射敏感性。结果表明，肿瘤病人皮肤成纤维细胞通过体外培养能够增殖传代，不同肿瘤病人皮肤成纤维细胞的放射敏感性存在明显的个体差异。

In order to test the radiosensitivity of normal skin tissure, we cultured cancer patient's skin tissure fibroblast, Surviving fraction experiment was employed to provide data for understanding of the different radiosensitivity among the cancer patients, Medthod: cancer patient's skin tissure fibroblast were cultured in vitro by the way of tissure attachment, cells wereirradiated by graded doses of γ-ray, cell dose response experiment was used to test the radiosensitivity of cell. Result: Cancer patient's skin fibroblast could be propagated and passaged by the method of culture in vitro. Radiosensitivity are different among the various cancer patient's skin tissure fibroblasts.