HLA-DPB1等位基因与中国人重症肌无力相关性研究

HLA DPB1 alleles associated with Myasthenia Gravis in Chinese patients

目的：探讨在中国汉族人群中重症肌无力（ＭＧ）与ＨＬＡＩ类基因的关联性。方法：随机抽取了５９例重症肌无力患者的外周血ＤＮＡ，用聚合酶链式反应限制性片段长度多态性（ＰＣＲＲＦＬＰ）方法，分析了其ＨＬＡＤＰＢ１座位的基因型，并与正常对照进行比较。结果：发现ＨＬＡＤＰＢ１０４０２与ＭＧ呈负相关（１１．８６％ｖｓ．２６．９２％；ｒ＝０．１８４３，Ｐｃ＝０．０２８９，ＰＦ＝０．２２０３）；１９０１与ＭＧ则呈正相关（１５．２５％ｖｓ．０．９６％；ｒ＝６．２０８１，Ｐｃ＜０．０１，ＥＦ＝０．０６９０）。结论：ＨＬＡＤＰＢ１座位在ＭＧ的发病过程中具有重要作用。

Objective:To investigate the association between Myasthenia Gravis (MG) and HLA class II genes in the Chinese Han population.Methods:HLA genotyping was done using polymerase chain reaction combined with restriction fragment length polymorphism( PCR PFLP).Results:Thirteen DPB1 alleles were detected in the 59 MG patients. The DPB1 allele, DPB1*1901 was positively associated with MG(15.25% vs. 0.96%; rr=6.208 1, Pc <0.01, EF=0. 069 0); and a negative association was found for DPB1*0402(11.86% vs. 26.92% ; rr=0.184 3, Pc =0.028 9, PF=0.220 3). Conclusion:HLA DPB1 locus was included in the progress of MG.