PCR结合悬滴法对女性生殖道感染的诊断价值

Diagnosis of Women Patients with Genital Tract by PCR Combined Drop hanging Method

对２５８例女性生殖道感染（典型滴虫、霉菌性阴道炎除外）患者，用ＰＣＲ技术检测ＮＧＨ、ＵＵ、ＣＴ，用悬滴法检测滴虫和霉菌。结果：病原体总检出率９３．８０％，单一病原体感染率４７．６７％，混合感染率４６．１２％。感染率以ＵＵ（５５．８１％）＞ＣＴ（４７．２９％）＞ＮＧＨ（４３．０２％）。认为两种方法结合应用灵敏度高、特异性强、操作时间短，可作为女性生殖道感染的常规检查方法，ＰＣＲ结合培养法可用以作为疗效观察和判断预后。对非典型脓性白带患者，应进行多指标检测。

women patients with genital tract infection except typical cases of trichomoniasis and colpitis mycotica were examined by PCR to detect NGH,UU,CT and by drop hanging method to detect trichomonds and fungi Results:The overall pathogen detected rate is 93 80%,single pathogen infective rate is 47 67% ,mixed infective rate is 46 12% Infective rates of UU, CT and NGH are 55 81%,47 29% and 43 02% respectively Two methods may be employed as routine clinical ways to detect pathogens in femal vaginitis,PCR may combine with traditional culture methods to extimate prognosis Various tests should be made on purulent discharges without typical symptoms