随机扩增多态DNA(RAPD)标记用于丁香品种遗传分析及品种分类(英文)

Using random amplified polymorphic DNA (RAPD) markers for lilac genetic analysis and classification in lilac cultivars

采用随机扩增多态ＤＮＡ（ＲＡＰＤ）标记分析了１５个丁香品种的ＤＮＡ扩增产物。研究选用了１６个随机引物，共扩增出９６条带，其中５５条带为可重复性条带，有价值条带大小多在５１７ｂｐ至１６３６ｂｐ之间。这些标记足以区分这些丁香品种。欧丁香（Ｓｙｒｉｎｇａｖｕｌｇａｒｉｓ）与Ｓ．×ｈｙａｃｉｎｔｈｉｆｌｏｒａ间的相似系数为６１．５％，欧丁香与Ｓ．×ｐｒｅｓｔｏｎｉａｅ间的相似系数为４７．２％，Ｓ．×ｈｙａｃｉｎｔｈｉｆｌｏｒａ与Ｓ．×ｐｒｅｓｔｏｎｉａｅ间的相似系数为４３．６％。结果表明，欧丁香与Ｓ．×ｈｙａｃｉｎｔｈｉｆｌｏｒａ亲缘关系最近。应用ＲＡＰＤ资料分析讨论了一些品种的起源。ＲＡＰＤ技术为丁香品种分类鉴定提供了可靠方法。

Random amplified polymorphic DNA (RAPD) markers generated by 16 arbitrary primers,were used to assess relationships among 15 lilac ( Syringa ) cultivars (18 accessions).A total of 96 bands were scored among which 55 bands were polymorphics.The informative bands were distributed between 517 bp to 1636 bp.These markers scored were sufficient to distinguish these cultivars.The genetic similarities were 61.5% between cultivars of Syringa vulgaris and S.× hyacinthiflora ,47.2% between cultivars of S.vulgaris and S.× prestoniae ,and 43.6% between ones of S.× hyacinthiflora and S.× prestoniae ,which showed that S.vulgaris was closely related to S.× hyacinthiflora .Origin of some cultivars was discussed on the basis of RAPD data.RAPD technique provides a simple and reliable alternative approach for identification of lilac cultivars.