大豆根瘤细菌周膜的冷冻复型研究

Freeze fracture study on peribacteroid membranes in soybean root nodules

用冷冻复型电镜技术研究了中国丰收１１号大豆根瘤中的细菌周膜。细菌周膜的断裂面上有颗粒状物质，但Ｐ面和Ｅ面有所不同，前者颗粒密度较大。即使都在Ｐ面或Ｅ面上，不同的细菌或同一细菌不同部位的颗粒密度也不一样。在细菌周膜与细菌细胞壁之间有一个环形腔隙，腔的大小随细菌和细菌部位不同而异。腔中不仅有泡状和管状结构，有时也有类寄主细胞质物质。细菌周膜表面有近似半球形或嵴形隆起，它们可能是腔中管泡状结构压迫细菌周膜外鼓所致。本文还讨论了细菌周膜断裂面上颗粒密度不同的原因及腔中管泡状结构的来源。

Peribacteroid membranes of chinese soybean ( Glycine max L.cv. Harvest 11) root nodules were studied by freeze fracture replica electron microscopic method.We found that there are particles on the fracture faces of peribacteroid membranes,but particle density on P face and E face is different,the former is quite abundant,the latter is less.Even if they all are on P face or E face,their density is also not same in different bacteroids or different parts of a bacteroid.There is a space between a peribacteroid membrane and a bacteroid cell wall,the size of spaces is variable in different bacteroids or the different parts of a bacteroid.There are not only tubular and vesicular structure but sometimes also some similar host cytoplasm material in the spaces.Some semicircle or ridge protruberances of the appear on the surfaces of peribacteroid membranes,they may result from the pressure of the tubular and vesicular structure in the spaces on peribacteroid membranes.This paper discussed the cause of distinction of the particle density and the orgin of tubular and vesicular structure in spaces.