用溴甲基五氟苯衍生气相色谱法测定葡萄浆果中的脱落酸和吲哚-3-乙酸

Determination of abscisic acid and indole 3 acetic acid of grape berry as the pentafluorobenzyl derivative with electron capture gas chromatography

ＡＢＡ和ＩＡＡ是葡萄浆果中的两个重要激素，用溴甲基五氟苯对其衍生、酯化成各具有５个氟的两种衍生物，这两种酯在气相色谱柱上分离效果良好，对电子检测器反应敏感。该方法操作简单。

Abscisic acid (ABA) and indole 3 acetic acid (IAA) are two kinds of very important plant hormones.They are converted in to pentafluorobenzyl esters by α bromo 2,3,4,5,6 pentafluorotoluene,the two esters generated are able to be simultaneously determined with electron capture gas chromatography,The method is simple and sensitive.