柄扁桃果实抑制物对小麦种子萌发时2种抗氧化酶活性的影响

Effecs of Prunus pedunculata Pall. Fruit Extracts on the Content of Dehydrogenase and Catalase during the Germination of Wheat Seeds

[目的]探讨柄扁桃果实提取物对小麦种子萌发过程中脱氢酶和过氧化氢酶活性的影响。[方法]通过分光光度计法和高锰酸钾滴定法分别测定小麦种子萌发过程中脱氢酶和过氧化氢酶的活性。[结果]柄扁桃内果皮粗提物在0.1g/ml时,小麦种子内脱氢酶含量最高,而果肉粗提物在0.1～0.15g/ml时,小麦种子内脱氢酶含量最高;内果皮粗提物在0.05g/ml时,小麦种子内过氧化氢酶含量最高,而果肉粗提物在0.10g/ml时,小麦种子内过氧化氢酶活性含量最高。[结论]柄扁桃的果肉和内果皮提取液对小麦种子萌发过程中的脱氢酶和过氧化氢酶含量均有影响,因此可以通过选择合适浓度的柄扁桃果实提取物来培养小麦种子。

[Objective] The aim was to study the effects of Prunus pedunculata Pall. fruit extracts on the content of dehydrogenase and catalase during the germination of wheat seeds. [Method] The UV spectrophotometry and the permanganimetric method was used to determine the content of dehydrogenase and catalase in wheat seeds respectively. [Result] It showed that the content of dehydrogenase in wheat seeds was highest with the 0.1 g/ml endocarp extracts and the 0.1-0.15 g/ml sarcocarp extracts treating the wheat seeds respectively,while the content of catalase was highest with the 0.05 g/ml endocarp extracts and the 0.1 g/ml sarcocarp extracts treating the wheat seeds respectively. [Conclusion] The content of dehydrogenase and catalase in wheat seeds all were affected by the Prunus pedunculata Pall. fruit extracts,therefore,the appropriate concentration of the Prunus pedunculata Pall. fruit extracts could be used to culture wheat seeds.