野生半夏不同生长期内γ-氨基丁酸含量的测定

Determination of γ-Aminobutyric Acid in Pinellia ternate (Thunb.) Breit. at Different Growth Stages

[目的]建立半夏[Pinellia ternate(Thunb.) Breit.]中γ-氨基丁酸含量的测定方法。[方法]采用薄层扫描法测定不同生长期半夏中γ-氨基丁酸含量。[结果]γ-氨基丁酸含量在0～1.0mg/ml范围内与峰面积积分值呈良好的线性关系(r=0.9981),平均加样回收率为95.6%,RSD为4.12%。5月上旬生长的半夏中γ-氨基丁酸的含量最高达0.143%。[结论]提取γ-氨基丁酸的野生半夏原料以5月上旬采收最佳。

[Objective]To establish a method for determing γ-aminobutyric acid in the Pinellia ternate （Thunb.）Breit..[Method] γ-Aminobutyric acid in the Pinellia ternate （Thunb.）Breit. at different growth stages was determined by TLC.[Result] The calibration curve showed good linearity in the range of 0-1.0 mg/ml （ r =0.998 1）,the average spike recovery was 95.6%,RSD was 4.12%. The content of γ-aminobutyric acid was 0.143% in early May.[Conclusion]The best time is in early May to collect Pinellia ternate （Thunb.）Breit..