摘要
通过ＰＣＲ方法扩增，用ｐＵＣ１１９和ｐＢｌｕｅｓｃｒｉｐｔＳＫ质粒载体分段克隆了山东省分离的ＳＪ１株的全病毒ＤＮＡ。
The genomic DNAof
chicken anemia virus (CAV) SJ1 strain, which was isolated from Shandong province in China,
was amplified by PCRwith the primers P 1,P 2 and P 3,P 4.Pland P 2 flank a 1500bp DNA
fragment containing the VP 2, VP 3 and the part of VP 1 genes. P 3 and P 4 flank a 800bp DNA
fragment containing the rest of RP 1 corresponding to the nucleotide sequence of Cux-1.
Endonuclease analysis of the PCR-amplified DNA with the enzymes AccI, BglⅡ, PvuⅡ,PstⅠ,
SacⅠ and HindⅢ showed that the restriction fragments of SJ1 strain is similar to that deduced
from the nucleotide sequence from Cux-1 strain. In addition, the DNA fragment was cloned into
the plasmid vectors of pUC119 and pBluescript SK respectively. It may be useful to molecular
biological study of CAV isolated from China.
出处
《生物技术》
CAS
CSCD
1999年第3期1-3,共3页
Biotechnology
