摘要
比较了５种细菌、４种酵母菌和鸡肝中双乙酰还原酶含量，其中粪肠杆菌（Ｅｎｔｅｒｏ－ｃｏｃｃｕｓｆａｅｃａｌｉｓ）ＡＳ１．５９５中含量较高，为９．６ＩＵ／ｇ湿菌体。并对ＡＳ１．５９５菌酶和鸡肝酶进一步经ＳｅｐｈａｄｅｘＧ－１００和ＤＺＡＥ柱分离纯化，提纯倍数分别为４５、８９３，最后收率分别为１８．５％、５．１％。
xperiments compared the
diacetyl reductase(DR)content of chick liver, bacter(five strains) and yeast (four strains), and DR
content of Enterococcus faecalis AS1.595 was highest(nearly 9.6 IU/g wet bacter). With sephadex
G_100 and DEAE cellulose 52, the DRs from AS1.595 and chick liver and were purified 45 folds
and 893 folds, and the final yields were 18.5%and 5.1% separately.
出处
《生物技术》
CAS
CSCD
1999年第3期12-14,共3页
Biotechnology
关键词
双乙酰还原酶
分离
纯化
diacetyl reductase,
isolation, purification
