摘要
探索出胶醋杆菌在摇瓶培养最适培养基成分为蛋白胨浓度１．０％、酵母膏０．５％、葡萄糖浓度２．０％、柠檬酸０．１１５％、乙醇１％、Ｎａ２ＨＰＯ４０．５％，ｐＨ６．０，１６０ｒ／ｍｉｎ的条件下进行摇瓶培养，细菌纤维素最大产量为７．５５ｇ／ｌ。
complex
medium was developed for the production of microbial cellulose by Acetobacter xylinum. The
optimum concentration of each nutrient for the production of microbial cellulose was determined
to be peptone 1%, yeast extract 0.5%, glucose 2%, Na 2HPO 4 1%, citric acid 0.115%, ethanol
1%. The maximal yield of cellulose productionis 7.55g/l at shaking speed 160 r/min, pH6.0. Cell
growth and the cellulose production in the developed medium under shakingflask culture was
also investigated.
出处
《生物技术》
CAS
CSCD
1999年第3期27-30,38,共5页
Biotechnology
关键词
胶醋杆菌
细菌纤维素
摇瓶培养
最适培养基
Acetobacter xylinum, bacterial cellulose, maximal yield,
shaking-flask culture
