一次PCR扩增单纯疱疹和巨细胞病毒基因的检测方法

PCR ASSAY FOR DETECTION OF HSV AND CMV IN ONCE PCR REACTION TUBE

宫内感染是影响优生优育质量的一个重要因素，由病毒引起的宫内感染主要有以下四类病原：ＨＳＶ、ＣＭＶ、ＴＯＸＯ和风疹病毒（ＲＶ），其中ＨＳＶ和ＣＭＶ的感染率较高。孕期这两种病原的感染会影响胎儿的正常发育，严重的可导致流产甚至胎儿畸形。目前已有针对这两种病...

wo mixed primer sets for HSV and CMV were used to specially amplify its target sequences in one PCR reaction tube. The results are in the line with that of one primer set PCR for each virus. Eight clinical urine samples were detected by this method. The reaction protocol, parameter of the cycles and the proportion of DMSO were evaluated for the optimization of PCR. This assay can be used to screen the HSV and CMV infection.