银染PCR-SSCP方法检测胃癌微小卫星DNA不稳定性

DETECTION OF MICROSATELLITE INSTABILITY BY SILVER STAINING PCR-SSCP IN HUMAN GASTRIC CARCINOMA

应用银染PCR-SSCP方法检测30例常规石蜡包埋胃癌组织第2、5、17号染色体4个位点的微小卫星DNA不稳定性(MIN)。结果发现11例阳性病例(36.7％),其中出现4、3、2个及单个位点异常分别为1(3％)、2(6％)、5(16.7％)和3例(10％)。4个位点中,以D5S107发生率最高(33.3％),其次为D2S123(30％)、D17S250(16.7％)、D2S119(12％)。提示MIN是胃癌病变中常见的分子遗传学事件,MIN的出现可能是胃癌发生的又一重要机制。银染PCR-SSCP是一简便、快速、灵敏、有效的检测MIN的方法。

Silver staining PCR-SSCP method was used to detect microsatellite instability (MIN)at 4 loci on chromosome 2, 5、17 in paraffin-embedded gastric carcinoma tissues. The abnormal shifting of the single-stranded DNA was identified in 11 out of 30 cases(36. 7%). Of these 11 tumors,1(3%)showed MIN at all 4 loci,2 tumors(6%)at 3 loci,5 tumors (16. 7%)at 2 loci,and 3 tumors(10%)at one locus. From these 4 loci, the detective rate of MIN was the hightest at D5S107(33. 3%),and it was 30%,16. 7%, 12% at D2S123.D17S250 and D2S119,respectively. The results indicate that MIN is a common molecular hereditary event in gastric carcinoma,and it may be one of the important mechanisms of gastric carcinogenesis. Silver staining PCR-SSCP is a convenient,rapid,sensitive and reliable method for detecting of MIN in gastric carcinoma.