苦参碱治疗长春瑞滨所致兔耳化疗性静脉损伤的实验研究

The Protection Effects of Matrine on Vinorelbine Tartrate Induced Venous Injury

目的探讨苦参碱对长春瑞滨导致的化疗性静脉损伤的治疗作用。方法建立长春瑞滨致兔耳静脉化疗性静脉损伤模型,分别采取苦参碱软膏涂抹和硫酸镁湿热敷治疗,于治疗后48h及7d分别观察静脉损伤情况,并取局部静脉及周围组织进行病理组织切片HE染色,评价静脉及周围组织的病理损伤愈合情况。结果治疗后48h,苦参碱组、硫酸镁组与模型组之间静脉组织病理损伤相比,苦参碱组在管腔充血、炎细胞浸润及血管周围出血等方面程度最轻(P<0.05);治疗后7d,苦参碱组在炎细胞浸润、血管周围出血等方面病理损伤程度最轻(P<0.05);苦参碱组静脉损伤消退时间最短(P=0.000)。结论苦参碱可用于治疗长春瑞滨所致的化疗性静脉损伤。

Objective To explore the mechanism of atherosclerosis of Rabbit induced by Hyperhomocysteinemia. Methods 44 New Zealand white rabbits were randomly divided into 4 groups： the control group （A group） were given standard feed; the model group （B group） took 1% methionine diet 100 g·d^-1 feeding; low -dose intervention group （C group） took 1% methionine diet 100g·d^-1 feeding ＋ Reduced Glutathione 15mg·d^-1 lintramuscularly; high- dose intervention group （D group） took 1% methionine diet 100g·d^-1 feeding ＋ Reduced Glutathione 30mg·d^-1intramusculady, at 0 and the 8th week, rabbit central ear artery blood were taken and plasma total homocysteine levels, serum total cholesterol, triglyceride, C - reactive protein, super - oxide dismutase were detected. Results Plasma Hcy and C - reactive protein were significantly higher than those in control group. （ P 〈 0.05 ）. SOD activity were significantly lower than those in control group （ P 〈 0.05）. Glutathione intervention could reduce the serum Hcy concentrations and raise SOD activity （P 〈 0.05 ）. Conclusion Glutathione can prevent the occurrence and development of atherosclerosis development by inhibiting the oxidative stress and inflammatory response.