摘要
蛋白质Ｃ端测序是蛋白质化学与分子生物学中一门有重要意义的技术，由Ｓｃｈｌａｃｋ和Ｋｕｍｐｆ于１９２６年提出的（异）硫氰酸法在该领域发展迅速并占主导地位．对（异）硫氰酸法的反应机理。
Protein C terminal sequence analysis is an important technique in protein chemistry and molecular biology. (iso)Thiocyanate approach appears to be the prevailing method since it was first described by Schlack and Kumpf in 1926. The chemical mechanism, the advances of (iso)thiocyanate approach and the preparation of amino acid thiohydantoins are reviewed and discussed in detail .
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第3期219-223,共5页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
关键词
蛋白质
C端序列
硫氰酸法
序列测定
protein C terminal sequencing, (iso)thiocyanate approach, activation, coupling, cyclization, cleavage, amino acid thiohydantoins
