对地贫红细胞的显微激光散射和图象分析

Microscope Laser Light Scattering Studies on Red Cells of Thalassemia with Image Processing and Analysis.

应用显微准弹性激光散射（ＭＱＬＳ）技术与显微生物医学图象分析技术对地中海贫血红细胞及胞内血红蛋白动态特性进行了研究．在实验中，比较了正常人及地贫患者红细胞胞内血红蛋白聚集体的平均流体力学半径、平均平动扩散系数及红细胞膜的搏动频率等动态特性参数，以及细胞的截面积、规化形状因子、长径、短径、灰度等图象分析数据，发现地贫红细胞的血红蛋白聚合物平均流体力学半径远远大于正常人红细胞的，其大小变异亦较正常人大，且其膜搏动频率也较为缓慢，细胞的截面积也变小．这反映了地贫红细胞内有较大的蛋白质聚合物存在和红细胞变形能力差的特性．研究还表明，显微准弹性激光散射技术结合图象分析技术，可使测量的可比性和准确性大大提高。

Microscopic quasi elastic laser light scattering technique and image processing and analysis technology were used to study thalassemia erythrocytes. The mean hydrodynamic radius and average diffusion coefficient of hemoglobin polymers in the intact red cells, the membrane motion frequency , cross area,regular form factor,longest axis,shortest axis and mean gray were measured for thalassemia erythrocytes and normal red cells. It is found that, compared with normal red cells, the mean hydrodynamic radius and its variance of thalassemia erythrocytes are larger, the membrane motion frequency is slower and the cross area of the cell is smaller. This suggests that thalassemia erythrocytes have large hemoglobin polymers and worse deformability.