摘要
用流式细胞计(flowcytometry,FCM)测定H2O2损伤后的红细胞(RBC)与IgG的结合能力,并直接检测细胞自发荧光的变化,以研究H2O2对RBC抗原性和脂质过氧化(LPO)荧光产物生成的影响.结果表明RBC抗原性和自发荧光变化与H2O2浓度和作用时间有关,抗原性变化对H2O2更敏感,比引起自发荧光明显增强所需H2O2浓度低两个数量级;
Using flow cytometry(FCM) to measure IgG binding to red blood cells(RBC) exposed to H 2O 2 and their autofluorescence directly, in order to study the effect of H 2O 2 on the antigenicity and the production of fluorescent product of LPO of RBC. The results showed that the changes of both antigenicity and autofluorescence were dependent on the H 2O 2 concentration and the time of RBCs exposed to H 2O 2, and the change of antiginicity was more susceptible to H 2O 2 than that of autofluorescence . At 10 2 fold lower H 2O 2 concentration than that made autofluorescence significantly increase, IgG bond to RBC. Furthermore, it was found that the changes of antigenicity and autofluorescence were related to forward scatter (FSC).
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第3期254-258,共5页
Progress In Biochemistry and Biophysics
关键词
红细胞
活性氧
细胞膜损伤
流式细胞计
抗原性
erythrocyte, flow cytometry, damage of cellular membrane,oxygen free radical,antiginicity