摘要
利用蛋白质结构同源模建并结合表观静电势分析，设计了拟具有生物学活性的蓖麻毒素Ａ链的突变体．将ＰＣＲ扩增的突变体基因，导入ｐＫＫ２２３３载体中，于大肠杆菌（Ｅ．ｃｏｌｉ）中获得高效、可溶性表达，而且。
Combined protein homologous modeling with analysis of electrostatic surface potential energy, mutant ricin toxin A chain, which was suggested to be bioactive, was designed. The gene of ricin toxin A chain was amplified by PCR and inserted in pKK223 3 to construct a prokaryotic expression vector, which was highly expressed in E.coli system. Moreover, the expression products showed obvious cytotoxicity, just in agreement with the prediction of modeling.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第3期270-272,294,共4页
Progress In Biochemistry and Biophysics
关键词
蓖麻毒素
A链突变体
基因表达
分子设计
protein homologous modeling,mutant ricin toxin A chain,gene expression, molecular design
