中国不同地区庚型肝炎病毒5′非编码区基因异质性分析

GENETIC HETEROGENEITY OF 5′UNTRANSLATED REGION OF HEPATITIS G VIRUSES ISOLATED FROM DIFFERENT REGIONS OF CHINA

为分析庚型肝炎病毒（ＨＧＶ）５′非编码区（５′ＵＴＲ）基因的异质性，确定中国不同地区ＨＧＶ主要流行株的特点及其分布，本研究对来自江西、湖南、河南、河北、甘肃等地９株ＨＧＶ５′ＵＴＲ区进行基因扩增与序列测定。通过对５′ＵＴＲ区１５４个核苷酸区段的序列进行分析，并与文献报导的其它２５株（１６株中国ＨＧＶ、９株国外代表株）相应区段进行比较与系统树分析，结果表明：（１）中国不同地区ＨＧＶ５′ＵＴＲ区基因存在一定异质性，但不存在明显的地区分布差异；（２）对３４株ＨＧＶ５′ＵＴＲ区的系统树分析，可分为３组，存在明显的地理分布特征；（３）绝大多数（除１株外）中国ＨＧＶ分离株皆分在第三组，并可分为二个亚组。

In order to investigate the genetic heterogeneity of 5′untranslated region (5′ UTR) of hepatitis G virus (HGV) and the main genotype of isolates in China, we have amplified and analyzed 5′UTR sequences of 9 virus strains, which were isolated from Jiangxi, Hunan, Henan, Hebei and Gansu Provinces of China. They were further compared with other 25 isolates (16 from China and 9 from other countries) in phylogenetic analyses. The results revealed two characteristics: (1) The genetic heterogeneity exists among different Chinese HGV isolates, but no difference in geographical distributions are demonstrated among them; (2) Phylogenetic analyses based on 5′UTR (159-312 nt) lead to suggestion that 34 HGV isolates are divided into three groups and display different geographical distributions. The majority (except one) of Chinese isolates belong to the group 3 of HGV and can be further divided into two subgroups.