柯萨奇B组病毒在Hep-2细胞内增殖特性的研究

Propagation Characteristics of Coxsackie B Virus in Hep 2 Cells

采用ＩＦＡ、ＲＴＰＣＲ法观察了柯萨奇Ｂ组病毒（ＣＢＶ）５型在Ｈｅｐ２细胞内的增殖动态。ＣＢＶ感染细胞后４ｈ可检出病毒ＲＮＡ，８ｈ可检出病毒抗原，１２ｈ可观察到细胞病变，２０ｈ可检出病毒颗粒。结果为ＣＢＶ增殖特性的研究提供了新的资料，并指出ＰＣＲ是监测ＣＢＶ增殖的敏感方法。

The dynamic change of coxsackie B virus (CBV) 5 type propagation was studied in Hep 2 cells by immunofluorescence assay, reverse transcription polymerase chain reaction and cytological methods. The results showed that CBV RNA could be detected at 4 hours in postinfection, viral antigen was positive at 8 hours and cytopathic effect could be found at 12 hours. The viral particles could be observed at 20 hours in postinfection. These results provided new data for observation of CBV propagation, also, it indicated that the reverse transcription polymerase chain reaction was a sensitive technique for monitoring CBV propagation.