大熊猫犬瘟热病毒融合蛋白基因3′端的序列测定

The Nucleotide Sequence of the 3′ End of Fusion Protein Gene of Canine Distemper Virus Giant Panda Strain

从死亡大熊猫的肝脏中直接提取细胞总ＲＮＡ，经反转录后，用犬瘟热病毒的两对引物，分两段扩增出融合蛋白基因３′端的片段。经序列分析表明：此片段的长度为６９３ｂｐ；此毒株在核苷酸和氨基酸水平与某疫苗弱毒株、Ｏｎｄｅｒｓｔｅｐｏｏｒｔ弱毒株、海豹瘟热病毒２型毒株和海豹瘟热病毒１型的同源性分别为９７．５％和９６．２％、８８．６％和９４．５％、９２．９％和９５．６％、６３．２％和７６．５％；大熊猫毒株与其它毒株融合蛋白３′端疏水性和二级结构均有一定差异；其非编码区比国外报道的Ｏｎｄｅｒｓｔｅｐｏｏｒｔ弱毒株多９个碱基，在非编码区最后５８个碱基，两毒株仅有１７个相同。

The cellular total RNA was isolated from livers of the died giant panda, then the templates were produced by reverse transcription reaction. Two couple primers were applied to amplify the 3′ end of fusion protein gene of canine distemper virus (CDV) giant panda strain. About 620 bp and 780 bp fragments were amplified. The fragments were purified and sequenced. There were 693 base pairs in the 3′ end of fusion protein gene of CDV giant panda strain. Its homology with CDV vaccine attenuated strain, CDV Onderstepoort attenuated strain, phocine distemper virus type 2 (PDV 2) and phocine distemper virus type 1 (PDV 1) in nucleotide and amino acid were 97.5% and 96.2%, 88.6% and 94.5%, 92.9% and 95.6%, 63.2% and 76.5%, respectively. The hydropathy profile and the second structure of the fusion protein gene in the 3′ end of CDV giant panda strain were different from other strains. The numbers of the non encoding region nucleotides of CDV giant panda strain were 9 nucleotides longer than CDV Onderstepoort strain in the same region. There were only 17 nucletides homology in the lastest 3′ end 58 bp between the two CDV strains.