尖吻蝮蛇出血毒素Ⅲ三维荧光光谱研究

Studies on the Three dimensional Fluorescence Spectra of Hemorrhagin Ⅲ from the Snake Venom of Agkistrodon acutus

蛇毒中含有多种金属蛋白质，它们具有不同的生物学活性，如抗凝血因子（ＡＣＦ）、糖苷水解酶（ＮＡＤａｓｅ）、纤溶组分（ＦＰ）及出血毒素等，它们的许多生物活性、荧光光谱和结构的性质等方面的研究已见报道［１］．徐洵等［２］从皖南尖吻蝮蛇蛇毒中分离出３种出血毒...

The fluorescence spectra changes of AaH Ⅲ in denaturants were studied by three dimensional fluorescence spectroscopy. The results showed that the emission bands of AaH Ⅲ remained at the same wavelength for the different excitation in 0 1% SDS solution, while red shift ocurred when deviated from the maximum excitation wavelength in 5 0 mol·L -1 guanidine HCl solution. It was conferred from the three dimensional fluorescence spectra that the Trp residues were partially located in the hydrophobic core of AaH Ⅲ molecule.