LC—MS／MS法测定牛肉中玉米赤霉醇的残留

Vestigital of Zeranol in Beef by Liquid Chromatography-Tandem Mass Spectrometry

目的：建立动物源性食品牛肉中玉米赤霉醇的残留分析方法。方法：采用叔丁基甲醚溶剂提取牛肉中玉米赤霉醇的残留，以乙腈-水（70：30）为流动相，流速0．8ml，进样量50μl，经高效液相色谱分离，以多反应监测（MRM）测定。结果：本方法在0．5—6．25ng／ml内线性良好（r=0．9998）；检出限0．2μg／kg；方法回收率为80．45％，RSD为3．21％。结论：本方法稳定、可靠、准确，可用于动物源性食品牛肉中玉米赤霉醇的残留分析方法。

Aim：To establish a method for determination the zeranol in beef .Methods： Methyl t-Butyl Ether was used for extract zeranol in beef. With mobile phase consisting of Aeetonitrile-water（70： 30）and eluted at flow rate of 0.8ml. min- 1 at 25℃ .inject volume 50 μl To Separate by HPLC, to analysis with MRM.Results The line ranges for zeranol were within 0.5-6.25ng/ml .the limit of detection was 0.2μg/kg.the average recorvery was80.45% （RSD3.21%）. Conclusion：This method could be used for the determineation of zeranol in beef, and stable,reliable and accurate.