青叶胆中总黄酮的提取工艺研究

Study on Extraction Process Of Total Flavonoids in Swertia mileensis T.N.Ho etW.L.Shi

目的：用正交试验法优选青叶胆中总黄酮的提取工艺。方法：以50g原药材的总黄酮提取量为评价指标，选择提取用水量（倍）、提取时间（h）、提取次数（次）为考察因素，采用正交试验法确定青叶胆中总黄酮的最佳提取工艺，采用分光光度法对干浸膏中总黄酮的含量进行了测定，检测波长490nm。结果：最佳提取工艺条件为提取所用水量为18倍，提取次数为2次，回流提取时间为3小时，芦丁在0．008-0．048mg范围之内呈良好的线性关系。平均回收率为99．72％，RSD为0．29％。结论：总黄酮正交提取工艺的研究，结果比较可靠，含量测定方法简便、准确，可用于青叶胆干浸膏中总黄酮的含量测定。

Objective To study the optimum extraction process of total flavonoids in Swertia mileensis T.N.Ho etW：L. Shi. Methods The extraction of total flavonoids was chosen as the assessment index. Volume of water,extracting time and times were the studing factors. The extraction process was optimumized with the orthogonal design using speetrophotometric to determine total flavonoids content at 490nm wavelength. Results The optimum ethanol extracting process conditions were as follows： adding 18 fold of water extraction two times, heating for 3 hours. The rutin standard sample showed a good 1 inear relationship at the range of 0.008--0.048mg. The average reeovery was 99.72%and RSD was 0.29%. Conclusion The optimum extradion process of total flavonoids in Swertia mileensis T. N.Ho etW.L.Shi.was reliable, easy and accurate which earl be used for the total flavonoids extraction of Swertia mileensis T.N.Ho etW.L.Shi.